# FACS (Flow Cytometry)

> **NIH NIH P30** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 2020 · $111,352

## Abstract

PROJECT SUMMARY – FLOW CYTOMETRY SHARED RESOURCE
The Flow Cytometry Shared Resource of the Albert Einstein Cancer Center (AECC) was established in 1980 to
provide investigators with convenient and affordable access to a broad spectrum of required analytical and
preparative flow cytometry technologies. Over the subsequent nearly 40 years, the facility has expanded its
dedicated laboratory space and staff, and added multiple new instruments and equipment upgrades. This
growth has been enabled by continuous support from Cancer Center Support Grant, and generous input of
funds and the College of Medicine. This facility furnishes AECC investigators with the majority of their on-site
access to high speed fluorescence activated cell sorting (FACS) and advanced multiparameter flow cytometric
analysis. The technologies provided are essential to the research of many AECC members, and cannot be
outsourced or obtained at other sites in any practical or cost effective way. At present, the facility supports four
high speed cell sorters, including two FACSAria sorters, and Beckman Coulter MoFlo XDP and Astrios EQ
sorters. These three sorting platforms provide a high level of versatility, allowing optimization of protocols for
isolation of rare and delicate cell types. The facility also provides access to a variety of analytical cytometers,
which range from relatively simple and user friendly to advance analyzers such as the Becton-Dickinson LSR II
with 14 color capability. Efforts are underway to add a new Cytek Aurora analyzer to the facility, which would
further expand analytical capability to greater than 20 fluorescent parameters per cell. An iCys laser scanning
cytometer is also housed and operated in the facility, providing a platform for coupled cytometric and image
data collection on individual cells in a high throughput manner. The facility also provides biosafety containment
for sorting cells from fresh human tissues. In addition to supporting an extensive range of advanced analytical
and preparative flow cytometry equipment, the facility provides technical expertise and training to guide AECC
investigators and their staff in the application of basic and advanced flow cytometry techniques. The facility
also organizes and supports seminars and demonstrations of new technologies to assure that AECC
investigators are well informed about newly emerging applications of FACS that can enhance their research.
The overall goal of the facility is to provide the most up to date cell sorting and analytical resources for a wide
range of cancer-relevant studies, and to deliver these in the most convenient and cost effective manner
possible.

## Key facts

- **NIH application ID:** 9998877
- **Project number:** 5P30CA013330-48
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** Steven A Porcelli
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $111,352
- **Award type:** 5
- **Project period:** 1997-06-01 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9998877

## Citation

> US National Institutes of Health, RePORTER application 9998877, FACS (Flow Cytometry) (5P30CA013330-48). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9998877. Licensed CC0.

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