# Human Correlation Core

> **NIH NIH U19** · SCRIPPS RESEARCH INSTITUTE, THE · 2020 · $287,374

## Abstract

Summary
The Human Correlation Core will determine whether the novel genes identified by Projects 1 and 2 of this U19
are relevant to human disease. During the preceding fifteen years, the investigators forming the U19 team
have identified and contextualized a large number of previously unknown immune regulatory genes, both by
forward genetics studies and/or systems biology approaches. In addition, systems approaches have been
used to place novel genes within immune regulatory networks. These have included the molecular pathways
involved in pathogen recognition, signal transduction, and gene regulation leading to appropriately tailored
responses to infection.
Over the years, this program has provided seminal information about multiple pathways that mediate
inflammation, regulate responses to infection, and underlie successful vaccination. Here the Core will test
these genes for relevance in human immunity.
The efforts of the Human Correlation Core will be greatly expanded by systems based network analysis
(Project 2) and molecular phenotyping (Signaling Core). The Aderem laboratory (Project 2) has established
several methods for global transcriptomic analysis of limiting samples including robust amplification protocols
for handling small quantities of mRNA and chromatin accessibility measures (ATAC-seq) that can interrogate
as few as 500 cells. During the previous funding period of this U19 program, the Signaling Core developed
single-cell mass cytometry (CyTOF) as well as methods to both efficiently implement this technology at high-
throughput and comprehensively analyze the resulting data. CyTOF permits the simultaneous measurement of
50-100 parameters including surface markers, cytokines and chemokines, and phosphorylated signaling
molecules at the single-cell level. Where appropriate, the Core will collaborate with the Aderem and Nolan
laboratories to apply these approaches to primary human cells. Since some of our technologies, for example
CRISPR/Cas9 gene editing, require large numbers of cells as starting material (it is particularly difficult to use
the technique in non-dividing myeloid cells) the Core has established new approaches to isolate large numbers
of macrophage lineage cells derived from CD34+ cord blood cells.

## Key facts

- **NIH application ID:** 9999448
- **Project number:** 5U19AI100627-09
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** Richard J Ulevitch
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $287,374
- **Award type:** 5
- **Project period:** 2012-09-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9999448

## Citation

> US National Institutes of Health, RePORTER application 9999448, Human Correlation Core (5U19AI100627-09). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9999448. Licensed CC0.

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