# Illuminating Druggable Dark Matter

> **NIH NIH U24** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2020 · $2,497,882

## Abstract

Project Summary
The goal of this project is to generate data and reagents that help uncover critical functions of the poorly
characterized members of ion channels. We prioritize mouse models, given the high value investigators
place on phenotypes observed for genetic perturbation. However, we focus on co-perturbation of ion
channel genes and their interacting genetic components as opposed to singly altering ion channel genes
in mouse models. This approach will validate our proteomics approaches in the most definitive manner-
in vivo. We see in vivo exploration as an essential step to evaluate ion channel function.
Our major aims include mapping ion channel interactions and complexes using a high-throughput
proteomics platform at UCSF. These data will be interrogated using integrative approaches established
by the Monarch Initiative, where biochemical interactions will be validated and prioritized for further
study. Another major aim is function-centric: we use mouse models to elucidation of human disease
mechanisms, where we embrace a genetic interaction scheme to uncover ion channel redundancy and
polygenic effects. Together these integrative approaches complement each other, specifically the in vivo
genetic interaction platform interrogates those genes identified from proteomics and bioinformatics
analysis. We generate critical reagents and data including antibodies and gene expression patterns.
Our broad goal is to identify ion channel phenotypes relevant to fundamental human disease. We bring
on board a number of ion channel experts and mouse phenotyping specialists who can accomplish this
major programs goals in an efficient manner.

## Key facts

- **NIH application ID:** 9999567
- **Project number:** 5U24DK116214-04
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** LILY JAN
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $2,497,882
- **Award type:** 5
- **Project period:** 2017-09-15 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9999567

## Citation

> US National Institutes of Health, RePORTER application 9999567, Illuminating Druggable Dark Matter (5U24DK116214-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9999567. Licensed CC0.

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