# Proteomic approaches and live-cell imaging reveal unique aspects of delivery of cellulose synthase complexes to the plasma membrane

> **NSF 01002526DB NSF RESEARCH & RELATED ACTIVIT** · Pennsylvania State Univ University Park (PA) · $1,206,583

## Abstract

Understanding how plant cells synthesize cellulose not only lays the scientific foundation for using genetic tools to modify plant cell walls for advances in sustainable energy but also provides insights into fundamental questions regarding how plant cells control expansion and shape. This project aims to utilize a combination of quantitative live-cell imaging, quantitative proteomics, and functional genetics to investigate how plant cells couple exocytosis and endocytosis to regulate the abundance of cellulose synthase at the plasma membrane—a process that remains largely unexplored. These findings will enhance knowledge of both cell wall biosynthesis and the mechanisms underlying plant cell growth. Furthermore, the project will create research opportunities for undergraduate students. By tailoring research projects to various undergraduate research programs, this initiative will attract talented students and prepare them for graduate education and academic careers. Additionally, the project will include a hands-on research workshop for local high school students and their teachers at Pennsylvania State University, aiming to inspire interest in the biological sciences.

The ability to produce renewable energy is crucial for both the economy and the environment, with cellulosic biomass expected to be a key source for biofuel production. Cellulose microfibrils are synthesized at the plasma membrane by a protein complex known as the cellulose synthase complex (CSC), which con

## Key facts

- **NSF award ID:** 2432499
- **Awardee organization:** Pennsylvania State Univ University Park (PA)
- **SAM.gov UEI:** NPM2J7MSCF61
- **PI:** Ying Gu
- **Primary program:** 01002526DB NSF RESEARCH & RELATED ACTIVIT
- **All programs:** NANOSCALE BIO CORE
- **Estimated total:** $1,206,583
- **Funds obligated:** $1,206,583
- **Transaction type:** Standard Grant
- **Period:** 07/01/2025 → 06/30/2029

## Primary source

NSF Award Search: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2432499

## Citation

> US National Science Foundation, Award 2432499, Proteomic approaches and live-cell imaging reveal unique aspects of delivery of cellulose synthase complexes to the plasma membrane. Retrieved via AI Analytics 2026-06-06 from https://api.ai-analytics.org/grant/nsf/2432499. Licensed CC0.

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