Chemicals control the social lives of animals, and some compounds need to balance multifunctional roles to perform effectively. The compound arginine vasotocin (AVT) is used for both osmotic balance and social communication. All animals rely on AVT, or a related hormone, for similar functions and use chemical communication to transmit social information between members of their own species. Thus, determining how AVT affects chemical communication will offer broad insights into the mechanisms governing social dynamics. Since environmental humidity impacts both AVT secretion and the persistence of chemical signals used in communication, the relationship between AVT and chemical communication could depend on humidity. As behavioral thermoregulators, lizards occupy places with varying humidities, and many species rely on chemical signaling for territorial maintenance. This project compares two species of Sceloporus Spiny lizards representing opposite extremes in habitat humidity to ask about the relative impacts of humidity on chemical communication. This project will train undergraduates to conduct vertebrate research using techniques in animal behavior, neuroscience, and chemical ecology in the field and lab. A research technician will gain extensive training in experimentation, lab management, field research, undergraduate mentorship, and research dissemination as preparation for a career in STEM. The chemical analysis pipeline created through this project will offer engaging