This project explores a unique type of fungus that parasitizes aspen trees. Known as the aspen bracket, this fungus has a remarkable trait: insects tend to avoid it. This behavior suggests that the fungus — either on its own or in partnership with the aspen tree — may produce substances that are toxic or unappealing to insects. By studying how the aspen bracket functions, researchers hope to discover new insect-repelling or insect-killing compounds that are both effective and environmentally friendly. These natural substances may work in ways not previously seen in fungi, offering new approaches to pest control. The findings could have valuable applications in agriculture, forestry, and public health. In addition to its scientific goals, the project has a strong educational mission: it will involve undergraduate and high school students in hands-on research. By engaging students and sharing results with both the public and the scientific community, the project aims to inspire broader participation in science, technology, engineering, and math (STEM) fields. The metabolic profile of the aspen bracket (Phellinus tremulae) will be characterized with respect to the content and origin of insecticidal or repellent compounds. First, the project will identify and characterize metabolites in the fungus. Both untargeted and targeted metabolomics will be conducted using liquid chromatography–mass spectrometry (LC-MS), and compounds of interest will be purified and tested on insects t