# Collaborative Research: Unveiling the molecular mechanism of feeding tube formation by root-knot nematodes: structural and functional insights

> **NSF 01002526DB NSF RESEARCH & RELATED ACTIVIT** · University of Georgia Research Foundation Inc (GA) · $255,932

## Abstract

There is an urgent agricultural need for novel approaches to combat the billions of dollars lost annually to the devastating root-knot nematode, a soilborne roundworm and global threat to food and fiber crop production. Sedentary adult nematode females, which require intensive nourishment for several weeks during the production of hundreds of eggs, feed from plant root cells transformed into giant-cells. Nematode feeding and survival rely on a unique nematode-derived structure called the feeding tube. While the feeding tube has been described at the ultrastructural level, its composition and mechanism of assembly remains an enigma. This project will resolve the mystery of how the root-knot nematode feeding tube is assembled and determine its composition using a combination of immunohistochemistry, structural biology, protein interaction, and gene silencing. The overall goal is to identify the nematode proteins involved in feeding tube formation and translate this knowledge to develop novel root-knot nematode resistance in crop plants. The project will partner with the State Botanical Garden of Georgia for public service and outreach, targeting K-5 students. Through a series of field trips and summer camp activities promoting hands-on activities and interactions with graduate and undergraduate students, the project will bring awareness to the hidden enemies lurking below ground that impact our food supply. This project will support undergraduate and graduate student training.

## Key facts

- **NSF award ID:** 2519785
- **Awardee organization:** University of Georgia Research Foundation Inc (GA)
- **SAM.gov UEI:** NMJHD63STRC5
- **PI:** Melissa G Mitchum
- **Primary program:** 01002526DB NSF RESEARCH & RELATED ACTIVIT
- **All programs:** ELEMENTARY/SECONDARY EDUCATION, UNDERGRADUATE EDUCATION, GRADUATE INVOLVEMENT
- **Estimated total:** $255,932
- **Funds obligated:** $255,932
- **Transaction type:** Standard Grant
- **Period:** 08/15/2025 → 07/31/2028

## Primary source

NSF Award Search: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2519785

## Citation

> US National Science Foundation, Award 2519785, Collaborative Research: Unveiling the molecular mechanism of feeding tube formation by root-knot nematodes: structural and functional insights. Retrieved via AI Analytics 2026-06-06 from https://api.ai-analytics.org/grant/nsf/2519785. Licensed CC0.

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