The Dynein 2025 meeting is an international conference dedicated exclusively to the study of dynein motor proteins. Dyneins are large proteins that act as cellular engines, driving directed movement along protein tracks called microtubules. They are essential for a wide range of processes within cells, such as transporting internal components and enabling the movement of structures like cilia and flagella. Held in Ann Arbor, Michigan, from July 22–24, 2025, this meeting provides a crucial forum for experts to discuss the latest discoveries and future directions in this rapidly advancing field. It offers a unique opportunity for researchers to share unpublished findings, initiate collaborations, and debate new models. A key focus is fostering the next generation of US-based scientists; students, post-doctoral fellows, and young faculty will present their work, gain visibility, and connect with established leaders in the field through presentations, poster sessions, and networking opportunities. This in-person gathering is the first since 2016, emphasizing vibrant, interactive discussions to drive the field forward. The Dynein 2025 conference focuses on recent, often unpublished, transformative advances in dynein biology, building on breakthroughs in cryo-EM, biophysics, and cell biology. The program will highlight new insights into dynein architecture, regulation, and diverse cellular roles. Key discussion areas include near-atomic resolution structures of different classes