The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project is to build an automated, miniaturized, gravity-agnostic system for DNA extraction and preparation for sequencing in austere environments. DNA sequencing is used for a variety of purposes in research and medicine – for example, diagnosis and treatment of diseases; monitoring of pathogens in water supplies and food; and to study the effects of various stressors on humans, crops, and even pets. The global DNA sequencing market size of $11B in 2022 is expected to grow to over $50B by 2032 indicating potential for growth and expansion of DNA sequencing technologies into untapped markets. This project is disrupting the market by developing a portable, automated system for extracting DNA from a variety of biosamples. Small academic and start-up labs and those focused on space research will be early market targets. This project expects to generate nearly $20M in revenue by year three of production. This Small Business Innovation Research (SBIR) Phase II project proposes to take a unique approach to technology development – by building the technology for space and microgravity, the most extreme environment. Currently, there are no commercial automated, miniaturized technology exists for use in space for DNA extraction and sample preparation. Space is a unique environment that can offer novel scientific insights. Availability of advanced tools in microgravity, such as the tec