This I-Corps project focuses on the development of a dynamic circulation system to improve accuracy and reliability of 3D bioprinting process as well as the functionality of fabricated tissues and organs that could be used for transplants. One major problem in 3D bioprinting is that the suspended living cells in the bioink often sink to the bottom and adhere with each other to form clusters with different shapes and sizes during printing. This leads to uneven cell placement, which lowers the quality and function of the printed tissues. This issue is especially important for creating transplantable organs, testing new medicines, and developing treatments tailored to individual patients. Current methods, such as active stirring or using special materials, can harm cells, require precise bioink preparation, and are difficult to use with multiple cell types. The solution is a new circulation system that helps prevent cells from sinking and clumping together in the bioink. A peristaltic pump is used to move the bioink from the bottom of the container back to the top, and the bioink is kept gently flowing and well mixed while the cells remain viable. This technology has the potential to improve human health, support medical research, and reduce the need for organ donors, contributing to the nation’s well-being and scientific progress. This I-Corps project utilizes experiential learning coupled with a first-hand investigation of the industry ecosystem to assess the translation po