This regulation establishes permanent tolerances for the insecticide spinosad (Factor A and Factor D). Factor A is 2-[(6-deoxy- 2,3, 4-tri-O-methyl-alpha-L-manno-pyranosyl)oxy]-13-[[5- (dimethylamino)-tetrahydro-6-methyl-2 H-pyran-2-yl]oxy]-9-ethyl- 2,3,3a,5a,5b,6,9,10,11,12,13,14,16a,6b-tetradecahydro-14-methyl-1 H-as- Indaceno [3,2-d]oxacyclododecin-7,15-dione. Factor D is 2-[(6-deoxy- 2,3,4-tri-O- methyl-alpha-L-manno-pyranosyl)oxy]-13-[[5- (dimethylamino)-tetrahydri-6-methyl-2H-pyran -2-yl]oxy]-9-ethyl- 2,3,3a,5a,5b,6,9,10,11,12,13,14,16a,16b-tetradecahydro-4,14-dimethyl- 1H-as-Indaceno[3,2-d]oxacyclododecin-7,15-dione. This regulation establishes tolerances for residues of spinosad in or on the raw agricultural commodities (RACs ), in or on barley, buckwheat, oats, and rye (grains) at 0.02 parts per million (ppm); pearl millet, proso millet, and amaranth (grains) at 1 ppm; teosinte and popcorn (grains) at 0.02 ppm; grass, forage, fodder and hay group; nongrass animal feed group at 0.02 ppm; turnip greens at 10 ppm; cilantro, and watercress at 8 ppm; tropical fruits (sugar apple, cherimoya, atemoya, custard apple, ilama, soursop, biriba, lychee, longan, spanish lime, rambutan, pulasan, papaya, star apple, black sapote, mango, sapodilla, canistel, mamey sapote, avocado, guava, feijoa, jaboticaba, wax jambu, starfruit, passionfruit, acerola, and white sapote) at 0.3 ppm; ti leaves at 10 ppm. Additionally, this rule establishes a tolerance for spinosad on pistachio at 0.02 ppm under conditional registration. These tolerances were requested by the Interregional Research Project (IR-4), Rutgers, the State University of New Jersey, 681 U.S. Highway #1 South, North Brunswick, NJ 08902-3390. Spinosad is manufactured by Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, IN 46268. The IR-4 requested these tolerances under the Federal Food, Drug, and Cosmetic Act, as amended by the Food Quality Protection Act of 1996.