This regulation amends the established tolerance for residues of the herbicide glyphosate in or on canola, seed at 20 parts per million (ppm) by changing the tolerance expression from the combined residues of glyphosate only, to the combined residues of glyphosate and N-acetyl-glyphosate (expressed as glyphosate equivalents). E.I. du Pont de Nemours and Company requested this change under the Federal Food, Drug, and Cosmetic Act (FFDCA).