Cancer Center Administration

NIH RePORTER · NIH · P30 · $395,000 · view on reporter.nih.gov ↗

Abstract

We propose to purify SARS-CoV-2 proteins to build a plate or filter-based array for rapid assessment of antibody responses. Proteins will be expressed in E. coli and purified as recombinant proteins. The arrays will initially be constructed manually using a vacuum blot system or ELISA plates. If arrays prove useful, a robotic system will be retooled. In addition to proteins already under study, we would expand our library to include all of the smaller open reading frames, especially proteins E and M, which are 100% conserved with SARS. The SARS-CoV-2 proteome can be covered in a 30 protein array. These arrays would then be used to ask novel questions about the development of the immune response in different patient cohorts, including cancer patients.

Key facts

NIH application ID
10164309
Project number
3P30CA060553-25S5
Recipient
NORTHWESTERN UNIVERSITY
Principal Investigator
Aleksandar Zafirovski
Activity code
P30
Funding institute
NIH
Fiscal year
2020
Award amount
$395,000
Award type
3
Project period
— → —