Project Summary PI: Francisco Fernandez-Lima This equipment supplement application requests funds to purchase a LEAP HDX Base System, and corresponding HDExaminer Data Analysis Software to support instrumental advances in tandem ion mobility – mass spectrometry (IMS/IMS-MS/MS) with the goal of providing needed complementary structural information to the middle-down and top-down strategies developed for the structural characterization of DNA binding proteins and ligand interaction dynamics under the aims of the PI’s NIGMS active grant R01-GM134247. This equipment supplement request will also streamline the application of solution HDX technology in tandem with unique middle- down and top-down IMS- MS strategies for several active-funded NIGMS investigators at FIU via the Advance Mass Spectrometry Facility, directed by the PI.