Project Summary/Abstract This application requests funding to purchase a new Bruker Avance NEO 600 MHz solution state NMR spectrometer with a 1.7 mm triple resonance micro-cryoprobe and a 60 place sample changer. The instrument will replace a defunct 600 MHz spectrometer that has been used for 15 years to develop and advance research programs in marine natural products discovery and biosynthesis, structural biology, drug design and synthesis, metabolomics, and systems biology. These research programs have discovered new drug leads, revealed new methods of synthesizing complex molecules, and aim to identify markers of human disease, and quantitatively describe life at the cellular level. NMR is essential to these projects and the requested instrument will restore the availability of instrument time. For the mass-limited samples these researchers deal with, a 1.7 mm micro-cryoprobe is essential as no other commercially available probe offers comparable mass-sensitivity. Furthermore, the inclusion of a sample changer with the requested instrument will enable research programs to expand into high throughput omics scale research.