A SARS-CoV-2 NFC ePAD Biosensor

NIH RePORTER · NIH · R01 · $58,053 · view on reporter.nih.gov ↗

Abstract

Proposed here is the development of an improved electrochemical sensor for detection of the SARS-CoV-2 nucleocapsid protein using thermoplastic electrodes (TPEs). The need for faster testing amid the COVID-19 pandemic brought about several alternatives to RT-qPCR testing for viral infections. However, current rapid tests are lacking in sensitivity (lateral flow assays and other paper based tests) or expensive (gold based electrochemical tests). TPEs solve both the sensitivity and cost issues and have been shown to be more robust than stencil printed carbon electrodes (SPCE). The TPE surfaces will be covalently modified using electroreduction of aryldiazonium salts followed by “click chemistry” to provide a platform for simple antibody crosslinking reactions. The fully characterized and functional sensor will then be the basis for future work integrating it into a microfluidic device for a full on-chip assay.

Key facts

NIH application ID
10635462
Project number
3R01EB031510-01S1
Recipient
COLORADO STATE UNIVERSITY
Principal Investigator
DAVID S DANDY
Activity code
R01
Funding institute
NIH
Fiscal year
2022
Award amount
$58,053
Award type
3
Project period
2021-09-13 → 2023-09-12