PROJECT SUMMARY The Human Immune Monitoring Center (HIMC) has been a comprehensive resource for immunological assays at Stanford for fifteen years. The HIMC Core will leverage this facility and its robust infrastructure to provide biobanking, assays, and data organization services to the CCHI projects. Coordinating with the Clinical Core, blood samples will be processed for PBMC, serum, RNA, and DNA, and biobanked according to optimized and standardized procedures. Together with the existing inventory of many thousands of specimens from previous CCHI and HIPC studies, these samples will be distributed to CCHI projects as needed, using an existing online portal to request specific samples, with approval from an oversight committee. State-of-the-art, standardized immune assays will also be applied to the CCHI samples, including CyTOF mass cytometry, multiplexed Luminex and Olink cytokine analysis, hemagglutinin inhibition, phospho-flow cytometry, and whole blood RNAseq, to provide comprehensive immunological data. As required for specific CCHI projects, custom Luminex panels and single-cell multi-omics assays (BD Rhapsody, Mission Bio Tapestri, Parse Evercode, and/or Fluent PIPseq) will be performed. Finally, the HIMC Core will integrate data from all standard HIMC assays with clinical and demographic metadata using the online database, Stanford Data Miner (SDM). Existing scripts will then allow formatting of data from SDM into ImmPort templates for samples, persons, and assay results. The HIMC Core will create a valuable database of clinical specimens and comprehensive immunological data, that will not only serve the needs of the CCHI U19 projects, but many other projects for years to come.