Abstract: Project Summary This grant proposal requests funding for a NovaSeq X Plus system manufactured by Illumina, San Diego, CA. This instrument will be incorporated into a successful Genomics facility, the Molecular Biology Core Facilities (MBCF) at the Dana-Farber Cancer Institute (DFCI). This core facility has provided molecular biology services, both genomic and proteomic, by chargeback since 1984. The NovaSeq X Plus will be used as a high impact expansion, complementary to existing Next-Generation sequencing services currently provided by the MBCF on two Illumina NextSeq 550s, 4 MiSeqs, a MiniSeq and a NovaSeq 6000. This expansion will allow cost effective and rapid turnaround time for whole genome sequencing, whole genome methylation profiling, single-cell genomics, and spatial transcriptomics applications. The increased capacity and substantially less expensive cost per basepair will increase the economic feasibility of much larger projects and improve access to cutting edge technology for smaller projects.