Project summary/abstract Using serial block-face scanning electron microscopy, we have produced the first adult mouse retinal volume that spans the entire depth of the retina, from photoreceptors to the nerve fiber layer at nanometer resolution to map mouse retinal cell types and their microcircuits. There are currently no other mouse retinal volumes that encompass both the inner and outer retina. Cellular ultrastructure and synaptic detail are excellent. Based on this volume, our goal is to provide the community with the most complete first-draft connectome of the mouse retina available anywhere within about a year. To achieve this, we propose to use Scalableminds Voxelytics services to stitch together and register the images, then segment the entire volume and detect ribbon and conventional synapses. These services will accelerate the availability of a fully segmented volume of the mouse retina that will greatly benefit ongoing retinal circuitry projects as well as facilitate new discoveries by the community.