Project Summary The goals of the Pathology and Bioinformatics Core are to act as the primary resource for biomaterials, histopathology expertise, tissue biomarker development, and bioinformatics support for the Program. This will provide Project Investigators with the necessary expertise and infrastructure to support the proposed individual projects and facilitate inter-project integration. The work of the Core will ensure quality control, biobanking, statistical analyses, expert opinion for study design, availability of equipment and resources to execute experimental approaches, and integration of data across different datasets and species for the individual Projects. The specific Aims of this Core are: 1) To provide acquisition, biobanking, clinical annotation, and histopathologic interpretative services for human and animal tissue samples. 2) To deploy existing and to develop novel tissue-based biomarker assays. 3) To provide bioinformatic support and interpretation of genomic and transcriptomic data. 4) To develop and provide methods for quantitative spatial analysis of multiplexed tissue biomarker data. Rigorous computational tools, analysis, and expertise will provide necessary resources to each individual Project in the Program, and overall integration of these data will develop a comprehensive, integrated model of local molecular and cellular tumor-immune signaling networks governing immune response and escape in melanoma.