ABSTRACT We are requesting an instrument supplement to purchase an Incucyte S3 Live-Cell Analysis System from Sartorius. Progress from the funded NIGMS R01 project for the past years has necessitated us implementing the real-time and quantitative analysis of genome maintenance mechanisms in live cells. The Incucyte S3 allows us to collect and analyze images of cell behaviors automatically and continuously in physiologically relevant conditions. The specific applications relevant to the current project include, but are not limited to, real-time cell cycle analysis, cellular proliferation, cell viability, and cell fate decision affected by the multiple mutants that we have generated and under chemotherapy. The ability to monitor and image a variety of cellular processes involved in DNA replication and stress responses under the real-time kinetics is crucial not only for the successful completion of the parent R01 award but also for the new trajectories of research with a versatile and powerful platform.