Project Summary I request funds to purchase several key components in order to add an advanced functionality of an existing live-cell imaging microscope system. Specifically, I propose to add an NSPARC (confocal multi-array detector) to the Nikon Eclipse Ti2 microscope system currently equipped for Total Internal Reflection Fluorescence (TIRF) microscopy. This would allow for low-light 3D detection of ultra-fine structural information beyond the resolution limit. This additional microscope capacity will allow for super-resolution imaging of subcellular dynamics in live samples of various thickness, including single cells and multicellular tissue samples. This equipment is needed to achieve goals of the parent grant with deep insights and a cutting-edge data quality.