The goal of this application is to request funding to purchase the Jess System from ProteinSimple for automated quantitative protein analysis. The Jess system is a capillary-based Western system that is gel free and blot free and which fully automates loading, size-based protein separation, data analysis and antibody probing, washing and detection. The Jess System executes protein analysis with high sensitivity, exceptional reproducibility, accurate protein quantification and rapid and high-throughput capabilities. Incorporating the Jess System into my research will improve my research program, enhance the Rigor and Reproducibility for studies outlined in the parental R01 award. Additionally, the Jess System will facilitate adherence to the data sharing policy of NIH.