This Phase I SBIR contract is in support of Development of novel In-vitro and In-vivo Models to support NeuroHIV Research. Upon the completion of Phase I, This contract aims to 1) Develop and create an integrated BBB tissue analog and incorporate a 3D micro-TEER system to assess the baseline of barrier integrity 2) Establish the co-culture of 3D BBB with organoidal-neurosphere, and validate the key gene expressions, BBB permeability, and neuron functions in our MPS platform 3) Validate the MPS platform can be used to study neuroinflammation and neuroimmune communication