Project Summary Hox genes serve as critical regulators of developmental processes. Disruption of their function during embryogenesis results in dramatic “homeotic” phenotypes where regions of the body are transformed from one identity to another. In humans, these disruptions can lead to malformation of the face, ears, limbs, and genitalia, as well as neural defects and cancer. In many animal genomes, the Hox genes are found in clusters: in vertebrates, these clusters are compact, while those of invertebrates are more loosely arranged or fragmented. While still poorly understood, the structure of the Hox cluster is hypothesized to be important in regulating their deployment. However, this is difficult to study in vertebrates as their genomes encode multiple Hox clusters that are the result of whole genome duplications. While invertebrates typically have a single complement of Hox genes, many invertebrate Hox clusters are disrupted, including those found in the classic invertebrate model systems like flies and nematodes. To address this deficit, we have developed resources and tools for studying cephalopod molluscs (squid and octopus), including chromosome-scale genome assemblies, extensive transcriptomics, and tools for gene manipulation. Through this work, we have found that cephalopods have a single, intact, but massively expanded Hox cluster. In fact, they encode the largest Hox clusters yet described – the squid Hox cluster is two orders of magnitude larger than those found in humans. Conservation of the Hox cluster in cephalopods is particularly striking given that their genomes are otherwise highly rearranged relative to other animals. Notably, we have found that cephalopod Hox genes exhibit the canonical, collinear nested domains of expression, suggesting that elements of the ancestral regulatory program are retained in cephalopods despite the dramatic increase in cluster size. Surprisingly, our preliminary knockout data suggest that loss of a Hox gene results